Khảo sát tác động điều hòa miễn dịch in vitro của một số dược liệu trên tế bào đơn nhân máu ngoại vi người

The immune system plays an essential role in safeguarding our body from surrounding detrimental factors in the environment. Nevertheless, when this system functions abnormally, it can result in immune-related diseases. Medicinal plants have been used widely in the treatment of immune-related diseases, yet little is known about their mechanisms of action. Therefore, the aim of this study was to investigate the in vitro immunomodulatory effect of some medicinal plants on human peripheral blood mononuclear cells (PBMCs) isolated from whole blood of healthy donors. MTT assay was used to evaluate the effect of eighteen crude ethanol extracts and fractions on PBMCs in vitro proliferation. The plant extract that showed the strongest inhibitory effect on PBMCs in vitro proliferation was chosen to evaluate the effect on CD3+CD4+ and CD3+CD8+ T lymphocytes. Results showed that the chloroform extract of Wedelia chinesis showed strongest inhibitory activity with an IC50 value of 16.1 ppm, exerting a decrease of 4.18 % in TCD3+CD4+ compared to untreated cells. In the conclusion, the chloroform extract of Wedelia chinensis had a potential of being used in the treatment of autoimmune diseases.
Keywords: Medicinal plants, peripheral blood mononuclear cells, immunomodulatory, in vitro.