XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG AMYGDALIN TRONG CAO HUYẾT PHỦ TRỤC Ứ BẰNG PHƯƠNG PHÁP HPLC – PDA

SUMMARY
Huyet phu truc u (HPTU) is a famous remedy of Chinese traditional medicine used for the treatment of cardiovascular diseases such as thrombosis, angina pectoris, myocardial infarction. To meet treatment needs nowadays, an extract was manufacturedfrom 11 medicinal plants as in HPTU remedy. Amygdalin, a primarily chemical component included in HPTU remedy could significantly decrease plasma viscosity, prolong the activation time of thromboplastin and reduce fibrinogen content, which prevents the condition of blood coagulation increase. Amygdalin is also an ingredient chosen as a marker to control the quality of dosage forms from HPTU remedy. The research was conducted to establish an HPLC method for determination of amygdalin in HPTU extract. The method was optimized as follows: Column – Phenomenex C18 (250 × 4.6 mm; 5 µm), temperature – 25 oC; Mobile phase – a mixture of methanol and water (21.5:78.5), in isocratic mode; Flow rate – 1,0 mL/minute; Injection volume – 20 µL; Detector – photodiode array (210 nm). The method was validated for the suitability of the system, specificity, linearity, repeatability, intermediate precision, accuracy (recovery rate = 98.1 – 101.1%) and quantification range (9.6 – 38.4 μg/mL). The procedure stretches 40 minutes for a run.
Keywords: Amygdalin, traditional medicine, determination, HPLC

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