Nghiên cứu xây dựng quy trình định lượng collagen trong thực phẩm chức năng bằng phương pháp LC-MS/MS

A LC-MS/MS was developed for determination of collagen through a hydrolyzed product
4-hydroxy-L-proline. The chromatography was performed as: column BEH Amide (100 x 2.1 mm;
1.7 µm); Mobile phase consists of acetonitrile / ammonium acetate 5 mM / formic acid 0.1 %
and ammonium acetate 5 mM formic acid 0.1 % / water in gradient mode; Detector- triple quadrupole mass spectrometer system using positive electrospray ionization and Multiple Reaction Monitoring mode. The recoveries range between 100.8 – 109.8 % with RSD 2.83 %. The validation results show that method is suitable for the indirect determination of collagen in materials and natural products.

Keywords: Hydroxyprolin, hydrolyzed collagen, LC-MS/MS.

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