ĐỊNH LƯỢNG FLAVONOID TOÀN PHẦN TRONG TAM GIÁC MẠCH (FAGOPYRUM ESCULENTUM) BẰNG PHƯƠNG PHÁP ĐO QUANG
Tác giả: NGUYỄN THỊ MINH DIỆP, NGUYỄN QUỐC TUẤN, NGÔ THỊ XUÂN THỊNH, ĐÀO VIỆT HƯNG, HÀ QUANG LỢI, HÀ THANH HÒA, HOÀNG ĐỨC LUẬN, NGÔ ĐỨC HUỆ, PHẠM QUỐC TUẤN
TÓM TẮT
Summary
Using a photometric method, which determined the content of total flavonoid in Fagopyrum esculentum and calculated by rutin. The regression equation of the method was y = 0.0109x – 0.0111 in the concentration range 10.0 – 100.0 µg/ml, a correlation coefficient of 0.9994 (R2 = 0.9994), and recovery rate 96.00 – 100.25 %. The results showed that the contents of flavonoids range from 7.15 – 7.61 % at different collection sites. The method is simple, fast, accurate, and suitable for the quantitation of the total flavonoid in Fagopyrum esculentum as well as the orientation for serving the standardization of the medicinal materials.
Keywords: Fagopyrum esculentum, total flavonoid, photometric method, rutin, quantitative analysis.
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