XÂY DỰNG MÔ HÌNH GÂY NÁM DA TRÊN THỎ VÀ ỨNG DỤNG KHẢO SÁT TÁC DỤNG CỦA CAO CHIẾT TỪ LÁ TÍA TÔ 

Summary

Background: Melasma is a common hyperpigmented disorder; the treatment is prolonged andusually less effective than expected. Therefore, development of safe and effective products from medicinal source is becoming emergent.
Objectives: This study aimed to establish a melasma model in rabbits and to investigate the preventive effect of Perilla leaf extract.
Materials and methods: Melasma formation was induced in New Zealand and British Giant rabbits by daily UVA radiation for 30 minutes combined to intramuscular injection of 5 mg/kg progesterone every other day. The response of the established model was evaluated by using the reference cream containing 4 % hydroquinone. Perilla leaves were extracted with 50 % ethanol by percolation method. The total extract was then liquid-liquid partitioned with ethyl acetate (EA) to obtain EA extract. This extract was daily applied at the concentration of 5 % and 10 % from the beginning of the experiment.
Results: The exposure to daily UV radiation combined to progesterone every other day in British Giant rabbits caused melasma areas which was markedly reduced by the local application of 4 % hydroquinone cream. EA extract significantly reduced the area of melasma as well as the melanin density distributed in the dermis of rabbit skin compared with untreated rabbits. The antipigmentation efficacy of 5 % and 10 % EA extract was not statistically significant.
Conclusion: The EA extract clearly exhibited its effective prevention of the melasma formation in the established model in British Giant rabbits.

Keyworks: Melasma, Perilla frutescens, hydroquinone 4 %.

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