Xây dựng quy trình phân tích đồng thời sáu hợp chất giảm glucose huyết ngụy tạo trong thuốc dược liệu và thực phẩm chức năng bằng phương pháp HPLC-PDA

An HPLC method was proposed to simultaneous determination of metformin, phenformin, glipizide, gliclazide, glibenclamide and glimepiride illegally added to traditional herbal medicines and food supplements as supported regime for diabetes mellitus. The above-mentioned chemical hypoglycemic agents are extracted by methanol, filtered by a membrane with pore size of 0.45 µm, and quantitatively determined by HPLC-PDA. The chromatographic conditions are as follows: InertSustain C18 column (250 x 4.6 mm; 5.0 µm), mixture of acetonitrile and 0.03% phosphoric acid as mobile phase in a gradient mode, detection wavelength at 230 nm, column temperature at 30oC, flow rate of 1.0 ml/minute, and injection volume of 10 µl. The HPLC method was validated basing on the guidelines of ICH and AOAC. The validation results prove the proposed method is reliable and practicable. The method was applied to control 10 available traditional herbal medicines and food supplements in Tay Ninh province. As the results, nine registered samples do not contain the above-mentioned chemical hypoglycemic agents, and one sample without registration is detected containing phenformin as an illegal adulterant.
Keywords: Metformin, phenformin, glipizide, gliclazide, glibenclamide, glimepiride, herbal medicines, food supplements.

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